Plasmid_Backbone

Part:BBa_K1391017:Design

Designed by: Jeremy Gam   Group: iGEM14_MIT   (2014-10-17)

MAV1212-RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1252
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2721
    Illegal XbaI site found at 2694
    Illegal SpeI site found at 2198
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 2682
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1252
    Illegal EcoRI site found at 2721
    Illegal SpeI site found at 2198
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 2682
    Illegal NotI site found at 402
    Illegal NotI site found at 1258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1252
    Illegal EcoRI site found at 2721
    Illegal BglII site found at 3182
    Illegal BamHI site found at 2205
    Illegal BamHI site found at 2700
    Illegal XhoI site found at 394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1252
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2721
    Illegal XbaI site found at 2694
    Illegal SpeI site found at 2198
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 2682
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1252
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2721
    Illegal XbaI site found at 1267
    Illegal XbaI site found at 2694
    Illegal SpeI site found at 2198
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 2682
    Illegal NgoMIV site found at 645
    Illegal NgoMIV site found at 926
    Illegal AgeI site found at 774
    Illegal AgeI site found at 1908
    Illegal AgeI site found at 2020
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2465
    Illegal BsaI.rc site found at 3083


Design Notes

Constructed through restriction-based cloning of PCR isolated RFP fragment into digested MAV1212 backbone.

Both PCR and MAV1212 fragment from Jeremy Gam.

Source

provided by the Weiss lab.

References